GeneJET Endo-Free Plasmid Maxiprep Kit from Thermo Fisher Scientific

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GeneJET Endo-Free Plasmid Maxiprep Kit

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Description

The GeneJET Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. Quantity: 10 preps.

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights
  • Efficient - high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
  • High concentration of DNA purified - > 400 ng/µL
    •Convenient - spin column format, centrifuge or vacuum protocols
  • Fast - procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
  • Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription
Includes
  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution
  • Endotoxin Binding Reagent
  • Wash Solution I (concentrated)
  • Wash Solution II (concentrated)
  • RNase A Solution
  • Elution Buffer (10 mM Tris-HCl, pH 8.5)
  • GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
  • Collection tubes (50 mL)
  Hazardous: No

Quality Control:
  • The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
  • The kit passes QC requirements if the purified plasmid DNA has an A260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
  • The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.
Storage Condition: Ambient   Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription